Fig. 10. Skeletal muscle responses to HMB in muscle from mice with ALD. (A). Representative immunoblots of incorporation of puromycin in ex vivo gastrocnemius muscle from PF mice and mALD treated with and without HMB. Densitometry of all blots in each lane. (B). Fractional and total synthesis rate of muscle protein in gastrocnemius muscle from PF mice and mALD above. (C). Representative immunoblots and densitometry of LC3 lipidation and Beclin 1 expression in mouse gastrocnemius muscle from mALD and PF mice with or without HMB. (D). Tissue weight (gm) of gastrocnemius and tibialis anterior muscles from mALD and PF mice treated with or without HMB. (E). Representative cross-sectional histological cryosections of gastrocnemius muscle oriented in the longitudinal direction from mALD and PF mice treated with or without HMB. All data expressed as mean±SD from n=4 PF mice and n= 6 mALD. * p<0.05; ** p<0.01, *** p<0.001. EtOH: ethanol; HMB b-hydroxy-b-methyl butyrate; UnT: untreated controls; PF: pair-fed; mALD: mouse model of ALD.